Hotspot

atpF

LSC · 12471-13778 · 1308 bp

Back to Osteomeles

Pi 0

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1308
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CTCAATACGCTCGCGGATAA	AGTCTATCTATAAGAAAGAGGATATCA	50.0	29.6	58.0	54.6	1440-1444 bp	1.0	40.3
atpF_p2	CTCAATACGCTCGCGGATAA	GTCTATCTATAAGAAAGAGGATATCA	50.0	30.8	58.0	53.3	1439-1443 bp	1.0	40.3
atpF_p3	CTCAATACGCTCGCGGATAA	AGTCTATCTATAAGAAAGAGGATATC	50.0	30.8	58.0	53.1	1440-1444 bp	1.0	40.3
atpF_p4	CTCAATACGCTCGCGGATAA	ATTAGTCTATCTATAAGAAAGAGGA	50.0	28.0	58.0	52.0	1443-1447 bp	1.0	40.3
atpF_p5	CTCAATACGCTCGCGGATAA	TTAGTCTATCTATAAGAAAGAGGAT	50.0	28.0	58.0	52.0	1442-1446 bp	1.0	40.3