Hotspot

accD

LSC · 60445-61971 · 1527 bp

Back to Osteomeles

Pi 0.000655

MarkerSeek score 54.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 1
pi: 0.000655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCCTCGTTATTAGTTACTAACCC	AACTACTTGATCACCACACA	45.8	40.0	58.2	54.2	1675 bp	1.0	40.0
accD_p2	CCCCTCGTTATTAGTTACTAACCC	AGTCAAAATCCATTCTTCGGA	45.8	38.1	58.2	55.2	1724 bp	1.0	40.0
accD_p3	CCCCTCGTTATTAGTTACTAACCC	TCCATTCTTCGGATTTTGACT	45.8	38.1	58.2	55.2	1716 bp	1.0	40.0
accD_p4	CCCCTCGTTATTAGTTACTAACCC	TCCATTCTTCGGATTTTGACTT	45.8	36.4	58.2	56.0	1716 bp	1.0	40.0
accD_p5	CCCCTCGTTATTAGTTACTAACCC	TCGGATTTTGACTTTGATTCCT	45.8	36.4	58.2	56.0	1708 bp	1.0	40.0