Hotspot

ndhF-rpl32

SSC · 116550-117660 · 1111 bp

Back to Osteomeles

Pi 0
MarkerSeek score 60.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1111
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 40.0 45.0 57.0 57.8 1288-1307 bp 1.0 71.5
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 40.0 45.0 57.0 57.8 1286-1305 bp 1.0 71.1
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 40.0 55.0 57.0 58.3 1275-1294 bp 1.0 70.2
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 40.0 45.0 57.0 57.6 1285-1304 bp 1.0 69.3
ndhF-rpl32_p5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 38.1 45.0 57.7 57.8 1289-1308 bp 1.0 69.2