Hotspot

rps7-rps12

IRb · 101742-101793 · 52 bp

Back to Osteomeles

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 52
variable_sites: 1
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTTCTTCTGCAGTACCTCGG	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.3	58.0	185 bp	1.0	74.4
rps7-rps12_p2	TTTCTTCTGCAGTACCTCGG	TCTGTGGAAAGCCGTATTCG	50.0	50.0	57.3	58.0	186 bp	1.0	74.4
rps7-rps12_p3	TTTTCTTCTGCAGTACCTCGG	CTGTGGAAAGCCGTATTCGA	47.6	50.0	58.0	58.0	186 bp	1.0	72.1
rps7-rps12_p4	TTTTCTTCTGCAGTACCTCGG	TCTGTGGAAAGCCGTATTCG	47.6	50.0	58.0	58.0	187 bp	1.0	72.1
rps7-rps12_p5	TTTCTTCTGCAGTACCTCGG	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.3	57.7	149 bp	1.0	71.9