Hotspot

ndhA

SSC · 120990-123171 · 2182 bp

Back to Osmanthus

Pi 0.00115

MarkerSeek score 55.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2182
variable_sites: 10
pi: 0.00115
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.6	57.8	2395-2413 bp	1.0	43.8
ndhA_p2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	39.1	50.0	56.5	57.8	2398-2416 bp	1.0	42.9
ndhA_p3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2327-2345 bp	1.0	40.9
ndhA_p4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2328-2346 bp	1.0	40.9
ndhA_p5	TCATGAACTCAGTTACCATAGGAA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.1	57.8	2398-2416 bp	1.0	40.9