Hotspot

rpl32-trnL(UAG)

SSC · 115093-115634 · 542 bp

Back to Osmanthus

Pi 0.00869

MarkerSeek score 59.50

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 542
variable_sites: 15
pi: 0.00869
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	GGATATGGGGCAGCGTTAAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	669-675 bp	1.0	81.3
rpl32-trnL(UAG)_p2	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	662-668 bp	1.0	80.9
rpl32-trnL(UAG)_p3	GGATATGGGGCAGCGTTAAA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	662-668 bp	1.0	80.8
rpl32-trnL(UAG)_p4	GGATATGGGGCAGCGTTAAA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	668-674 bp	1.0	80.7
rpl32-trnL(UAG)_p5	GGATATGGGGCAGCGTTAAA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	667-673 bp	1.0	80.7