Hotspot

ndhF-rpl32

SSC · 114452-114930 · 479 bp

Back to Osmanthus

Pi 0.00724

MarkerSeek score 60.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 479
variable_sites: 15
pi: 0.00724
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TTTAACGCTGCCCCATATCC	50.0	50.0	58.0	58.0	661-666 bp	1.0	82.1
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TTTAACGCTGCCCCATATCC	45.0	50.0	58.0	58.0	659-664 bp	1.0	82.0
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TTTAACGCTGCCCCATATCC	45.0	50.0	58.0	58.0	660-665 bp	1.0	82.0
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	AAATGCTTTTAACGCTGCCC	50.0	45.0	58.0	57.9	672-673 bp	0.9	76.5
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	AAATGCTTTTAACGCTGCCC	45.0	45.0	58.0	57.9	670-671 bp	0.9	76.5