Hotspot

ndhC-trnM(CAU)

LSC · 52394-54328 · 1935 bp

Back to Osmanthus

Pi 0.00207

MarkerSeek score 61.90

Primer available yes

Label ndhC-trnM(CAU)

Feature Metadata

label_name: ndhC-trnM(CAU)
feature_type: igs
region: LSC
length_bp: 1935
variable_sites: 13
pi: 0.00207
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM(CAU)_p1	GGGGCTAAAATCCCGGAAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	1934-2092 bp	1.0	80.6
ndhC-trnM(CAU)_p2	GGGGCTAAAATCCCGGAAAT	AGGTACCATTGACTCCGGTA	50.0	50.0	57.9	57.5	1995-2153 bp	1.0	79.5
ndhC-trnM(CAU)_p3	GGGGCTAAAATCCCGGAAAT	TAGGTACCATTGACTCCGGT	50.0	50.0	57.9	57.5	1996-2154 bp	1.0	79.5
ndhC-trnM(CAU)_p4	GGGGCTAAAATCCCGGAAAT	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	1933-2091 bp	1.0	78.5
ndhC-trnM(CAU)_p5	TTCTCCGGCCCTTTCCTAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.4	58.4	1955-2113 bp	1.0	77.9