Hotspot

rps2-rpoC2

LSC · 17427-17631 · 205 bp

Back to Osmanthus

Pi 0.0108

MarkerSeek score 59.00

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 6
pi: 0.0108
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	375-379 bp	1.0	79.3
rps2-rpoC2_p2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	369-373 bp	1.0	79.2
rps2-rpoC2_p3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.8	57.8	372-376 bp	1.0	78.7
rps2-rpoC2_p4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	376-380 bp	1.0	78.1
rps2-rpoC2_p5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.3	57.8	373-377 bp	1.0	77.9