Hotspot

atpA-atpF

LSC · 12749-12823 · 75 bp

Back to Osmanthus

Pi 0.0265

MarkerSeek score 65.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 8
pi: 0.0265
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TGTTGGGAGCGATGAAAGAA	50.0	45.0	58.0	57.4	207-224 bp	1.0	82.5
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	ATATTGGCATGTTGGGAGCG	50.0	50.0	58.0	58.7	216-233 bp	1.0	81.4
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	ATGTTGGGAGCGATGAAAGA	50.0	45.0	58.0	57.2	208-225 bp	1.0	80.7
atpA-atpF_p4	ATGCCATCGCCTACTTGAAG	CATGTTGGGAGCGATGAAAG	50.0	50.0	58.0	57.2	209-226 bp	1.0	80.3
atpA-atpF_p5	CAATGCCATCGCCTACTTGA	TGTTGGGAGCGATGAAAGAA	50.0	45.0	58.3	57.4	209-226 bp	1.0	80.2