Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9417-10115 · 699 bp

Back to Osmanthus

Pi 0.00535
MarkerSeek score 50.50
Primer available yes
Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name
trnS(GCU)-trnG(GCC)
feature_type
igs
region
LSC
length_bp
699
variable_sites
12
pi
0.00535
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCU)-trnG(GCC)_p1 CAATCCGACGCTTTAGTCCA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 798-828 bp 1.0 62.8
trnS(GCU)-trnG(GCC)_p2 AATCCGACGCTTTAGTCCAC AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 797-827 bp 1.0 62.8
trnS(GCU)-trnG(GCC)_p3 ATCAACGGAACCGGAAAGAG AGACCGAAAGACCCTTTAACT 50.0 42.9 57.9 56.6 862-892 bp 1.0 61.9
trnS(GCU)-trnG(GCC)_p4 CATCAACGGAACCGGAAAGA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 863-893 bp 1.0 61.1
trnS(GCU)-trnG(GCC)_p5 TCATCAACGGAACCGGAAAG AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 864-894 bp 1.0 61.1