Hotspot

rps15

SSC · 125572-125835 · 264 bp

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Pi 0.0341

MarkerSeek score 57.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 9
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGCATTGATGGGTGATGAGG	TGTATCCCCCGTTGCAAATT	50.0	45.0	57.9	57.8	826-830 bp	1.0	82.0
rps15_p2	GGTGATGAGGGCCCATATTG	TGTATCCCCCGTTGCAAATT	55.0	45.0	58.1	57.8	816-820 bp	1.0	81.8
rps15_p3	TAACTGTTCGGTTTTCGGCA	TGTATCCCCCGTTGCAAATT	45.0	45.0	58.0	57.8	939-943 bp	1.0	81.6
rps15_p4	AGTCTGTGCGCCAATATCTG	TGTATCCCCCGTTGCAAATT	50.0	45.0	58.1	57.8	1145-1149 bp	1.0	81.1
rps15_p5	GCTCTGGTCCATTTACGGTT	TGTATCCCCCGTTGCAAATT	50.0	45.0	57.9	57.8	1020-1024 bp	1.0	80.9