Hotspot

trnL-UAG-ccsA

SSC · 116463-116626 · 164 bp

Back to Osbeckia

Pi 0.058

MarkerSeek score 75.70

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 164
variable_sites: 8
pi: 0.058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATTGAAACGATCGAAAATGA	50.0	30.4	58.0	55.8	271-297 bp	1.0	46.3
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATTGAAACGATCGAAAATGA	50.0	30.4	58.0	55.8	270-296 bp	1.0	46.3
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGATCGAAAATGA	50.0	30.4	57.9	55.8	261-287 bp	1.0	46.3
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	ACAATTGAAACGATCGAAAATGA	55.0	30.4	57.8	55.8	263-289 bp	1.0	46.3
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	ACAATTGAAACGATCGAAAATGA	55.0	30.4	57.8	55.8	262-288 bp	1.0	46.3