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rps8-rpl14

LSC · 82024-82368 · 345 bp

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Pi 0.0814

MarkerSeek score 72.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 28
pi: 0.0814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTACAATTCCCATCCCGCC	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	733 bp	1.0	83.1
rps8-rpl14_p2	ATTACAATTCCCATCCCGCC	TGTCGCTGTGATCAAGGATG	50.0	50.0	58.0	58.0	918 bp	1.0	82.7
rps8-rpl14_p3	ATTACAATTCCCATCCCGCC	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	991 bp	1.0	82.5
rps8-rpl14_p4	CCCGCCTAAAATTCTCGGAA	TGTCGCTGTGATCAAGGATG	50.0	50.0	58.0	58.0	904 bp	1.0	82.5
rps8-rpl14_p5	CCCGCCTAAAATTCTCGGAA	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	977 bp	1.0	82.3