Hotspot

petD-rpoA

LSC · 79380-79578 · 199 bp

Back to Osbeckia

Pi 0.041

MarkerSeek score 74.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 8
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CATTTCGTCGTCCAGTAGCA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.0	57.1	340-346 bp	1.0	67.8
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	283-289 bp	1.0	67.8
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	284-290 bp	1.0	67.8
petD-rpoA_p4	TTGATTGGTACTGTGGTGGC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	308-314 bp	1.0	67.4
petD-rpoA_p5	CGTCCAGTAGCAACAACTGT	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	332-338 bp	1.0	66.9