Hotspot

psbE-petL

LSC · 65835-67001 · 1167 bp

Back to Osbeckia

Pi 0.0328

MarkerSeek score 72.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 38
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGGTTCGCCTCAGTCAATTA	45.0	50.0	58.0	57.6	1401-1403 bp	1.0	79.4
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GGGTTCGCCTCAGTCAATTA	50.0	50.0	57.8	57.6	1313-1315 bp	1.0	78.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GGGTTCGCCTCAGTCAATTA	55.0	50.0	58.2	57.6	1308-1310 bp	1.0	78.1
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	GGGTTCGCCTCAGTCAATTA	50.0	50.0	58.4	57.6	1306-1308 bp	1.0	76.9
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	GGGTTCGCCTCAGTCAATTA	50.0	50.0	58.6	57.6	1307-1309 bp	1.0	75.1