Hotspot

trnD-GUC-trnY-GUA

LSC · 31279-31716 · 438 bp

Back to Osbeckia

Pi 0.0374
MarkerSeek score 71.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
438
variable_sites
16
pi
0.0374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 499-509 bp 1.0 77.8
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 498-508 bp 1.0 77.8
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 520-530 bp 1.0 76.7
trnD-GUC-trnY-GUA_p4 TTTATTCCGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 552-562 bp 1.0 76.5
trnD-GUC-trnY-GUA_p5 TTTATTCCGTCGGGACTGAC GGCAATATGTCTACGCTGGT 50.0 50.0 57.3 57.8 551-561 bp 1.0 76.5