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atpH-atpI

LSC · 14283-15179 · 897 bp

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Pi 0.0325

MarkerSeek score 74.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 28
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.0	57.9	950-973 bp	1.0	83.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.0	58.1	962-985 bp	1.0	83.7
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	980-1003 bp	1.0	83.4
atpH-atpI_p4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	992-1015 bp	1.0	83.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.2	57.9	951-974 bp	1.0	82.7