Hotspot

trnR-UCU-atpA

LSC · 10059-10477 · 419 bp

Back to Osbeckia

Pi 0.0569

MarkerSeek score 80.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 23
pi: 0.0569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACCGAGGAAGCAGAA	47.6	50.0	57.3	57.9	512-516 bp	1.0	71.6
trnR-UCU-atpA_p2	ACAAAGAAAGCGTCCATTGTC	CATTCACCGAGGAAGCAGAA	42.9	50.0	57.4	57.9	571-575 bp	1.0	71.5
trnR-UCU-atpA_p3	ACAAAGAAAGCGTCCATTGTC	AAGACATTCACCGAGGAAGC	42.9	50.0	57.4	57.9	575-579 bp	1.0	71.5
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AAGACATTCACCGAGGAAGC	47.6	50.0	57.3	57.9	516-520 bp	1.0	71.5
trnR-UCU-atpA_p5	ACAAAGAAAGCGTCCATTGT	CATTCACCGAGGAAGCAGAA	40.0	50.0	56.2	57.9	571-575 bp	1.0	69.8