Hotspot

trnS-GCU-trnR-UCU

LSC · 8115-9986 · 1872 bp

Back to Osbeckia

Pi 0.0307

MarkerSeek score 70.60

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1872
variable_sites: 57
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.0	58.0	1971-1985 bp	1.0	83.0
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.0	58.0	1970-1984 bp	1.0	83.0
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.2	58.0	2025-2039 bp	1.0	81.7
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	ACGAACTTGCGTCCAATAGG	50.0	50.0	58.0	58.3	1972-1986 bp	1.0	80.8
trnS-GCU-trnR-UCU_p5	AATCCGACGCTTTAGTCCAC	ACGAACTTGCGTCCAATAGG	50.0	50.0	58.0	58.3	1971-1985 bp	1.0	80.8