Hotspot

rpoC2

LSC · 25062-29603 · 4542 bp

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Pi 0.0108
MarkerSeek score 44.60
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4542
variable_sites
171
pi
0.0108
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 GTTTTGTCGGCATCCAACTG ACTTTTGGAAGACCTTGCGT 50.0 45.0 57.9 57.9 611-632 bp 1.0 87.6
rpoC2_p2 TGCAGTCGCGTATGAAATGA ACTTTTGGAAGACCTTGCGT 45.0 45.0 58.0 57.9 1074-1095 bp 1.0 86.0
rpoC2_p3 TGCAGTCGCGTATGAAATGA ACCATGAACAGTTGCTCCTG 45.0 50.0 58.0 58.1 979-1000 bp 1.0 86.0
rpoC2_p4 TGCAGTCGCGTATGAAATGA TGACCATGAACAGTTGCTCC 45.0 50.0 58.0 58.1 981-1002 bp 1.0 86.0
rpoC2_p5 TGCAGTCGCGTATGAAATGA GGAAACCCGCACTTTTGATG 45.0 50.0 58.0 57.9 1333-1354 bp 1.0 84.9