Hotspot

psaJ-rpl33

LSC · 65312-65773 · 462 bp

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Pi 0.0183
MarkerSeek score 48.90
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
462
variable_sites
25
pi
0.0183
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GGTTTGGGGTTTTAGCAGGT TAACATCTTTCCCCTTGGCC 50.0 50.0 58.3 57.5 546-568 bp 0.954545 79.6
psaJ-rpl33_p2 TGGTTTGGGGTTTTAGCAGG TAACATCTTTCCCCTTGGCC 50.0 50.0 58.3 57.5 547-569 bp 0.954545 79.5
psaJ-rpl33_p3 TATCTCTCTGTAGCACCCGT TAACATCTTTCCCCTTGGCC 50.0 50.0 57.3 57.5 580-602 bp 1.0 78.1
psaJ-rpl33_p4 ATCTCTCTGTAGCACCCGTG TAACATCTTTCCCCTTGGCC 55.0 50.0 58.9 57.5 579-601 bp 0.954545 74.5
psaJ-rpl33_p5 GGTTTGGGGTTTTAGCAGGT ACATCTTTCCCCTTGGCCAT 50.0 50.0 58.3 59.3 544-566 bp 0.954545 73.3