Hotspot

ndhC-trnV-UAC

LSC · 50115-50904 · 790 bp

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Pi 0.0168
MarkerSeek score 55.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
790
variable_sites
44
pi
0.0168
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 749-835 bp 1.0 56.7
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 750-836 bp 1.0 54.9
ndhC-trnV-UAC_p3 GGCTTGCTATTATTAGAAATGCCC GGTTCGAATCCGTATAGCCC 41.7 55.0 58.3 57.9 781-867 bp 1.0 53.4
ndhC-trnV-UAC_p4 GCTTGCTATTATTAGAAATGCCCA GGTTCGAATCCGTATAGCCC 37.5 55.0 57.7 57.9 780-866 bp 1.0 53.0
ndhC-trnV-UAC_p5 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 748-834 bp 1.0 52.7