Hotspot

atpI-atpH

LSC · 31579-32404 · 826 bp

Pi 0.0103

MarkerSeek score 57.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 29
pi: 0.0103
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	537-895 bp	1.0	80.9
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	604-962 bp	1.0	80.8
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	559-917 bp	1.0	80.8
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	544-902 bp	1.0	80.6
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	617-975 bp	1.0	80.3