Hotspot

psbM-petN

LSC · 17107-17869 · 763 bp

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Pi 0.024
MarkerSeek score 67.80
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
763
variable_sites
49
pi
0.024
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 AAAACAGCCAGCCAAAATGA TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.7 58.2 817-886 bp 0.909091 73.0
psbM-petN_p2 AAAACAGCCAGCCAAAATGA GTAGTATGGGGGAGGAGTGG 40.0 60.0 56.7 58.3 761-830 bp 0.909091 72.3
psbM-petN_p3 AAAACAGCCAGCCAAAATGA TAGTATGGGGGAGGAGTGGA 40.0 55.0 56.7 58.4 760-829 bp 0.909091 71.6
psbM-petN_p4 AAACAGCCAGCCAAAATGAT TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.5 58.2 816-885 bp 0.909091 71.2
psbM-petN_p5 AACAGCCAGCCAAAATGATT TAGTAAGTCTCGCTTGGGCT 40.0 50.0 56.5 58.2 815-884 bp 0.909091 71.2