Hotspot

trnY-GUA-trnD-GUC

LSC · 16185-16547 · 363 bp

Back to Oryza

Pi 0.0317
MarkerSeek score 65.00
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
363
variable_sites
41
pi
0.0317
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 CTGGTTCAAATCCAGCTCGG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.9 58.5 389-421 bp 1.0 80.9
trnY-GUA-trnD-GUC_p2 TCTACGCTGGTTCAAATCCA CGGTGCTCTGACCAATTGAA 45.0 50.0 56.9 58.5 395-427 bp 1.0 78.9
trnY-GUA-trnD-GUC_p3 TGGTTCAAATCCAGCTCGG CGGTGCTCTGACCAATTGAA 52.6 50.0 57.8 58.5 388-420 bp 1.0 78.2
trnY-GUA-trnD-GUC_p4 GTCTACGCTGGTTCAAATCCA CGGTGCTCTGACCAATTGAA 47.6 50.0 58.3 58.5 396-428 bp 1.0 77.6
trnY-GUA-trnD-GUC_p5 TGTCTACGCTGGTTCAAATCC CGGTGCTCTGACCAATTGAA 47.6 50.0 58.3 58.5 397-429 bp 1.0 77.6