Hotspot

trnT-GGU-trnE-UUC

LSC · 15444-15966 · 523 bp

Back to Oryza

Pi 0.0185
MarkerSeek score 52.20
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
523
variable_sites
34
pi
0.0185
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 579-592 bp 1.0 73.8
trnT-GGU-trnE-UUC_p2 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 600-613 bp 1.0 73.7
trnT-GGU-trnE-UUC_p3 TGGTAAGGCATAAGTCATCGG TGTCCTGAACCACTAGACGA 47.6 50.0 57.5 57.7 584-597 bp 1.0 72.9
trnT-GGU-trnE-UUC_p4 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 605-618 bp 1.0 72.8
trnT-GGU-trnE-UUC_p5 GGTAAGGCATAAGTCATCGGT GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 604-617 bp 1.0 72.8