Hotspot

trnG-UCC-trnfM-CAU

LSC · 12490-12922 · 433 bp

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Pi 0.0169
MarkerSeek score 54.10
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
433
variable_sites
26
pi
0.0169
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 483-555 bp 1.0 85.5
trnG-UCC-trnfM-CAU_p2 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.1 58.3 498-570 bp 1.0 84.9
trnG-UCC-trnfM-CAU_p3 CGTAGCGAGCGTAGTTCAAT CGAGGCTCATAACCTTGAGG 50.0 55.0 58.2 57.8 487-559 bp 1.0 84.7
trnG-UCC-trnfM-CAU_p4 ATATAGGACGTAGCGAGCGT CGAGGCTCATAACCTTGAGG 50.0 55.0 58.2 57.8 495-567 bp 1.0 84.6
trnG-UCC-trnfM-CAU_p5 CCCAGGCGGATATACCCTAT CGAGGCTCATAACCTTGAGG 55.0 55.0 57.8 57.8 530-590 bp 0.909091 81.1