Hotspot

rps16-trnQ-UUG

LSC · 5607-6666 · 1060 bp

Back to Oryza

Pi 0.0272
MarkerSeek score 65.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1060
variable_sites
107
pi
0.0272
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAATCGATCGTGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 698-1156 bp 1.0 91.2
rps16-trnQ-UUG_p2 CCACAATCGATCGTGTCCTT TGACAGGCATAACATCCACG 50.0 50.0 58.0 58.0 1218-1676 bp 1.0 89.9
rps16-trnQ-UUG_p3 CCACAATCGATCGTGTCCTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1267-1725 bp 1.0 89.4
rps16-trnQ-UUG_p4 TGGTGGGTAGCAAAATCCAC TGACAGGCATAACATCCACG 50.0 50.0 58.1 58.0 1234-1692 bp 1.0 89.3
rps16-trnQ-UUG_p5 TGGTGGGTAGCAAAATCCAC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 1283-1741 bp 1.0 88.8