primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 CCACAATCGATCGTGTCCTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 15.0 16.9 0.086246 5607 6666 698-1156 1.000000 91.2 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 CCACAATCGATCGTGTCCTT TGACAGGCATAACATCCACG 20 20 50.0 50.0 58.0 58.0 15.0 0.0 0.032910 5607 6666 1218-1676 1.000000 89.9 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CCACAATCGATCGTGTCCTT CTTACAGCAGCTTGCCAAAC 20 20 50.0 50.0 58.0 57.9 15.0 0.0 0.066239 5607 6666 1267-1725 1.000000 89.4 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 TGGTGGGTAGCAAAATCCAC TGACAGGCATAACATCCACG 20 20 50.0 50.0 58.1 58.0 7.4 0.0 0.104892 5607 6666 1234-1692 1.000000 89.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 TGGTGGGTAGCAAAATCCAC CTTACAGCAGCTTGCCAAAC 20 20 50.0 50.0 58.1 57.9 7.4 0.0 0.138222 5607 6666 1283-1741 1.000000 88.8 trnG-UCC-trnfM-CAU_p1 trnG-UCC-trnfM-CAU 1 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 20 20 50.0 55.0 58.1 57.8 0.0 16.0 0.313942 12490 12922 483-555 1.000000 85.5 trnG-UCC-trnfM-CAU_p2 trnG-UCC-trnfM-CAU 2 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 20 20 50.0 55.0 58.1 58.3 0.0 0.0 0.367933 12490 12922 498-570 1.000000 84.9 trnG-UCC-trnfM-CAU_p3 trnG-UCC-trnfM-CAU 3 CGTAGCGAGCGTAGTTCAAT CGAGGCTCATAACCTTGAGG 20 20 50.0 55.0 58.2 57.8 0.0 16.0 0.407386 12490 12922 487-559 1.000000 84.7 trnG-UCC-trnfM-CAU_p4 trnG-UCC-trnfM-CAU 4 ATATAGGACGTAGCGAGCGT CGAGGCTCATAACCTTGAGG 20 20 50.0 55.0 58.2 57.8 3.2 16.0 0.425345 12490 12922 495-567 1.000000 84.6 trnG-UCC-trnfM-CAU_p5 trnG-UCC-trnfM-CAU 5 CCCAGGCGGATATACCCTAT CGAGGCTCATAACCTTGAGG 20 20 55.0 55.0 57.8 57.8 0.0 16.0 0.407462 12490 12922 530-590 0.909091 81.1 trnfM-CAU-trnT-GGU_p1 trnfM-CAU-trnT-GGU 1 CCTCAAGGTTATGAGCCTCG CCACTGAGTTAAAAGGGC 20 18 55.0 50.0 57.8 52.9 0.0 0.0 7.362166 12997 15371 2210-2474 1.000000 48.3 trnfM-CAU-trnT-GGU_p2 trnfM-CAU-trnT-GGU 2 CCTCGTGAGCTACCAAACTG CCACTGAGTTAAAAGGGC 20 18 55.0 50.0 58.3 52.9 0.0 0.0 7.416157 12997 15371 2195-2459 1.000000 48.3 trnfM-CAU-trnT-GGU_p3 trnfM-CAU-trnT-GGU 3 CAAGGTTATGAGCCTCGTGA CCACTGAGTTAAAAGGGC 20 18 50.0 50.0 57.3 52.9 0.0 0.0 7.803913 12997 15371 2207-2471 1.000000 48.3 trnfM-CAU-trnT-GGU_p4 trnfM-CAU-trnT-GGU 4 TCAAGGTTATGAGCCTCGTG CCACTGAGTTAAAAGGGC 20 18 50.0 50.0 57.3 52.9 0.0 0.0 7.803913 12997 15371 2208-2472 1.000000 48.3 trnfM-CAU-trnT-GGU_p5 trnfM-CAU-trnT-GGU 5 TATGAGCCTCGTGAGCTACC CCACTGAGTTAAAAGGGC 20 18 55.0 50.0 58.7 52.9 0.0 0.0 7.811411 12997 15371 2201-2465 1.000000 48.3 trnT-GGU-trnE-UUC_p1 trnT-GGU-trnE-UUC 1 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 20 20 45.0 50.0 56.6 57.7 0.0 0.0 1.653235 15444 15966 579-592 1.000000 73.8 trnT-GGU-trnE-UUC_p2 trnT-GGU-trnE-UUC 2 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 20 20 45.0 55.0 56.6 58.3 0.0 0.0 1.635085 15444 15966 600-613 1.000000 73.7 trnT-GGU-trnE-UUC_p3 trnT-GGU-trnE-UUC 3 TGGTAAGGCATAAGTCATCGG TGTCCTGAACCACTAGACGA 21 20 47.6 50.0 57.5 57.7 0.0 0.0 1.750547 15444 15966 584-597 1.000000 72.9 trnT-GGU-trnE-UUC_p4 trnT-GGU-trnE-UUC 4 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 21 20 47.6 55.0 57.5 58.3 0.0 0.0 1.732397 15444 15966 605-618 1.000000 72.8 trnT-GGU-trnE-UUC_p5 trnT-GGU-trnE-UUC 5 GGTAAGGCATAAGTCATCGGT GCTGCCTCCTTGAAAGAGAG 21 20 47.6 55.0 57.5 58.3 0.0 0.0 1.734033 15444 15966 604-617 1.000000 72.8 trnY-GUA-trnD-GUC_p1 trnY-GUA-trnD-GUC 1 CTGGTTCAAATCCAGCTCGG CGGTGCTCTGACCAATTGAA 20 20 55.0 50.0 58.9 58.5 0.0 7.1 1.387412 16185 16547 389-421 1.000000 80.9 trnY-GUA-trnD-GUC_p2 trnY-GUA-trnD-GUC 2 TCTACGCTGGTTCAAATCCA CGGTGCTCTGACCAATTGAA 20 20 45.0 50.0 56.9 58.5 0.0 7.1 1.618630 16185 16547 395-427 1.000000 78.9 trnY-GUA-trnD-GUC_p3 trnY-GUA-trnD-GUC 3 TGGTTCAAATCCAGCTCGG CGGTGCTCTGACCAATTGAA 19 20 52.6 50.0 57.8 58.5 0.0 7.1 1.725375 16185 16547 388-420 1.000000 78.2 trnY-GUA-trnD-GUC_p4 trnY-GUA-trnD-GUC 4 GTCTACGCTGGTTCAAATCCA CGGTGCTCTGACCAATTGAA 21 20 47.6 50.0 58.3 58.5 0.0 7.1 1.781973 16185 16547 396-428 1.000000 77.6 trnY-GUA-trnD-GUC_p5 trnY-GUA-trnD-GUC 5 TGTCTACGCTGGTTCAAATCC CGGTGCTCTGACCAATTGAA 21 20 47.6 50.0 58.3 58.5 0.0 7.1 1.781973 16185 16547 397-429 1.000000 77.6 trnD-GUC-psbM_p1 trnD-GUC-psbM 1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 20 20 50.0 40.0 58.5 56.7 5.4 2.0 1.775052 16622 17001 544-1070 0.909091 65.4 trnD-GUC-psbM_p2 trnD-GUC-psbM 2 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 20 20 50.0 40.0 58.5 56.5 5.4 2.0 2.006868 16622 17001 546-1072 0.909091 63.5 trnD-GUC-psbM_p3 trnD-GUC-psbM 3 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 20 20 50.0 40.0 58.5 56.5 5.4 2.0 2.006868 16622 17001 545-1071 0.909091 63.5 trnD-GUC-psbM_p4 trnD-GUC-psbM 4 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 20 21 50.0 38.1 58.5 57.4 5.4 2.0 2.034560 16622 17001 544-1070 0.909091 63.3 trnD-GUC-psbM_p5 trnD-GUC-psbM 5 TTCAATTGGTCAGAGCACCG TTTGGCTGGCTGTTTTTACA 20 20 50.0 40.0 58.5 56.3 5.4 2.0 2.128044 16622 17001 540-1066 0.909091 62.6 psbM-petN_p1 psbM-petN 1 AAAACAGCCAGCCAAAATGA TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.7 58.2 0.0 0.0 1.449068 17107 17869 817-886 0.909091 73.0 psbM-petN_p2 psbM-petN 2 AAAACAGCCAGCCAAAATGA GTAGTATGGGGGAGGAGTGG 20 20 40.0 60.0 56.7 58.3 0.0 0.0 1.579440 17107 17869 761-830 0.909091 72.3 psbM-petN_p3 psbM-petN 3 AAAACAGCCAGCCAAAATGA TAGTATGGGGGAGGAGTGGA 20 20 40.0 55.0 56.7 58.4 0.0 0.0 1.669873 17107 17869 760-829 0.909091 71.6 psbM-petN_p4 psbM-petN 4 AAACAGCCAGCCAAAATGAT TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.5 58.2 0.0 0.0 1.680884 17107 17869 816-885 0.909091 71.2 psbM-petN_p5 psbM-petN 5 AACAGCCAGCCAAAATGATT TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.5 58.2 0.0 0.0 1.680884 17107 17869 815-884 0.909091 71.2 trnC-GCA-rpoB_p1 trnC-GCA-rpoB 1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 18444 19548 1167-1242 1.000000 82.7 trnC-GCA-rpoB_p2 trnC-GCA-rpoB 2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 18444 19548 1166-1241 1.000000 82.7 trnC-GCA-rpoB_p3 trnC-GCA-rpoB 3 AAAGGATTTGCAGTCCCCTG TCCCTCATTTCCATTCCGGA 20 20 50.0 50.0 58.1 58.4 14.6 0.0 0.480541 18444 19548 1150-1225 1.000000 82.1 trnC-GCA-rpoB_p4 trnC-GCA-rpoB 4 AAAGGATTTGCAGTCCCCTG TTCCCTCATTTCCATTCCGG 20 20 50.0 50.0 58.1 57.6 14.6 0.0 0.506504 18444 19548 1151-1226 1.000000 81.9 trnC-GCA-rpoB_p5 trnC-GCA-rpoB 5 AAAGGATTTGCAGTCCCCTG TCCGGGTATTGTGGACATTC 20 20 50.0 50.0 58.1 57.3 14.6 0.0 0.773724 18444 19548 1168-1243 1.000000 79.7 rpoC2_p1 rpoC2 1 GTTTTGTCGGCATCCAACTG ACTTTTGGAAGACCTTGCGT 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.103259 28351 28470 611-632 1.000000 87.6 rpoC2_p2 rpoC2 2 TGCAGTCGCGTATGAAATGA ACTTTTGGAAGACCTTGCGT 20 20 45.0 45.0 58.0 57.9 15.7 0.0 0.055979 28351 28470 1074-1095 1.000000 86.0 rpoC2_p3 rpoC2 3 TGCAGTCGCGTATGAAATGA ACCATGAACAGTTGCTCCTG 20 20 45.0 50.0 58.0 58.1 15.7 0.0 0.101606 28351 28470 979-1000 1.000000 86.0 rpoC2_p4 rpoC2 4 TGCAGTCGCGTATGAAATGA TGACCATGAACAGTTGCTCC 20 20 45.0 50.0 58.0 58.1 15.7 0.0 0.106977 28351 28470 981-1002 1.000000 86.0 rpoC2_p5 rpoC2 5 TGCAGTCGCGTATGAAATGA GGAAACCCGCACTTTTGATG 20 20 45.0 50.0 58.0 57.9 15.7 0.0 0.067770 28351 28470 1333-1354 1.000000 84.9 atpI-atpH_p1 atpI-atpH 1 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 20 20 50.0 45.0 57.5 57.8 26.3 3.2 0.731910 31579 32404 537-895 1.000000 80.9 atpI-atpH_p2 atpI-atpH 2 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 20 20 50.0 55.0 57.5 58.2 26.3 0.0 0.717204 31579 32404 604-962 1.000000 80.8 atpI-atpH_p3 atpI-atpH 3 TAGCCGCAGCCTATATAGGT GCCAATCCAGCAGCAATAAC 20 20 50.0 50.0 57.5 57.8 26.3 0.0 0.741275 31579 32404 559-917 1.000000 80.8 atpI-atpH_p4 atpI-atpH 4 TAGCCGCAGCCTATATAGGT ATAACGGAAGCAGCAGCAAT 20 20 50.0 45.0 57.5 58.2 26.3 3.2 0.771708 31579 32404 544-902 1.000000 80.6 atpI-atpH_p5 atpI-atpH 5 ATTTTTGCAACGTTAGCCGC GCAGTACCTTGACCAACTCC 20 20 45.0 55.0 58.6 58.2 16.5 0.0 0.775939 31579 32404 617-975 1.000000 80.3 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 22 20 36.4 55.0 56.1 57.9 0.0 0.0 3.962644 50115 50904 749-835 1.000000 56.7 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 22 20 36.4 55.0 56.1 58.3 0.0 22.8 4.192025 50115 50904 750-836 1.000000 54.9 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 GGCTTGCTATTATTAGAAATGCCC GGTTCGAATCCGTATAGCCC 24 20 41.7 55.0 58.3 57.9 11.3 0.0 4.361932 50115 50904 781-867 1.000000 53.4 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 GCTTGCTATTATTAGAAATGCCCA GGTTCGAATCCGTATAGCCC 24 20 37.5 55.0 57.7 57.9 0.0 0.0 4.407348 50115 50904 780-866 1.000000 53.0 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 21 20 38.1 55.0 54.6 57.9 0.0 0.0 4.464233 50115 50904 748-834 1.000000 52.7 atpB-rbcL_p1 atpB-rbcL 1 TGATCGATACGCCCTGTACT AGTTTCTGTTTGTGGTGACA 20 20 50.0 40.0 58.0 55.3 0.0 0.0 2.686327 53850 54652 878-898 1.000000 64.8 atpB-rbcL_p2 atpB-rbcL 2 GGGAAAAGTGACATCCAGCA AGTTTCTGTTTGTGGTGACA 20 20 50.0 40.0 58.1 55.3 4.8 0.0 2.753684 53850 54652 912-932 1.000000 64.0 atpB-rbcL_p3 atpB-rbcL 3 GGGGGAAAAGTGACATCCAG AGTTTCTGTTTGTGGTGACA 20 20 55.0 40.0 58.2 55.3 4.8 0.0 2.823960 53850 54652 914-934 1.000000 63.5 atpB-rbcL_p4 atpB-rbcL 4 CCAGCACGGGTCCAATAATT AGTTTCTGTTTGTGGTGACA 20 20 50.0 40.0 58.2 55.3 0.0 0.0 2.900676 53850 54652 898-918 1.000000 62.9 atpB-rbcL_p5 atpB-rbcL 5 TGATCGATACGCCCTGTACT TGCTTTAGTTTCTGTTTGTGGTG 20 23 50.0 39.1 58.0 58.0 0.0 0.0 3.030256 53850 54652 884-904 1.000000 62.0 rbcL-accD_p1 rbcL-accD 1 GGCTTCAGGGGGTATTCATG CCATTGCAATTGCCGGAAAT 20 20 55.0 45.0 58.0 58.0 0.0 26.9 0.040121 56087 57112 2011-2103 1.000000 88.2 rbcL-accD_p2 rbcL-accD 2 GGCTTCAGGGGGTATTCATG TGCGCCTATTTCGAATCCAA 20 20 55.0 45.0 58.0 58.0 0.0 4.2 0.044376 56087 57112 1640-1708 1.000000 88.2 rbcL-accD_p3 rbcL-accD 3 GAGCTGTATTTGCGAGGGAA CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 0.0 26.9 0.051179 56087 57112 2384-2476 1.000000 87.5 rbcL-accD_p4 rbcL-accD 4 AAGAGCTGTATTTGCGAGGG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 0.0 26.9 0.056842 56087 57112 2386-2478 1.000000 87.5 rbcL-accD_p5 rbcL-accD 5 GAGCTGTATTTGCGAGGGAA TGCGCCTATTTCGAATCCAA 20 20 50.0 45.0 58.0 58.0 0.0 4.2 0.055434 56087 57112 2013-2081 1.000000 86.7 petA-psbJ_p1 petA-psbJ 1 TGAAAAGGTTCAATTGTACGA ACGGTTCATATTCTGGATTGGG 21 22 33.3 45.5 53.7 58.2 5.1 0.0 7.490155 61126 62121 1003-1075 0.954545 47.2 petA-psbJ_p2 petA-psbJ 2 TGAAAAGGTTCAATTGTACGA CGGTTCATATTCTGGATTGGGT 21 22 33.3 45.5 53.7 58.2 5.1 0.0 7.490155 61126 62121 1002-1074 0.954545 47.2 petA-psbJ_p3 petA-psbJ 3 AGAAACAGTTTGAAAAGGTTCA ACGGTTCATATTCTGGATTGGG 22 22 31.8 45.5 54.6 58.2 2.3 0.0 7.572616 61126 62121 1013-1085 0.954545 47.2 petA-psbJ_p4 petA-psbJ 4 AGAAACAGTTTGAAAAGGTTCA CGGTTCATATTCTGGATTGGGT 22 22 31.8 45.5 54.6 58.2 2.3 0.0 7.572616 61126 62121 1012-1084 0.954545 47.2 petA-psbJ_p5 petA-psbJ 5 TGAAAAGGTTCAATTGTACGAA CGGTTCATATTCTGGATTGGGT 22 22 31.8 45.5 54.5 58.2 5.1 0.0 7.652093 61126 62121 1002-1074 0.954545 47.2 psaJ-rpl33_p1 psaJ-rpl33 1 GGTTTGGGGTTTTAGCAGGT TAACATCTTTCCCCTTGGCC 20 20 50.0 50.0 58.3 57.5 0.0 0.0 0.813049 65312 65773 546-568 0.954545 79.6 psaJ-rpl33_p2 psaJ-rpl33 2 TGGTTTGGGGTTTTAGCAGG TAACATCTTTCCCCTTGGCC 20 20 50.0 50.0 58.3 57.5 0.0 0.0 0.814289 65312 65773 547-569 0.954545 79.5 psaJ-rpl33_p3 psaJ-rpl33 3 TATCTCTCTGTAGCACCCGT TAACATCTTTCCCCTTGGCC 20 20 50.0 50.0 57.3 57.5 0.0 0.0 1.256039 65312 65773 580-602 1.000000 78.1 psaJ-rpl33_p4 psaJ-rpl33 4 ATCTCTCTGTAGCACCCGTG TAACATCTTTCCCCTTGGCC 20 20 55.0 50.0 58.9 57.5 0.0 0.0 1.417991 65312 65773 579-601 0.954545 74.5 psaJ-rpl33_p5 psaJ-rpl33 5 GGTTTGGGGTTTTAGCAGGT ACATCTTTCCCCTTGGCCAT 20 20 50.0 50.0 58.3 59.3 0.0 6.1 1.575564 65312 65773 544-566 0.954545 73.3 ndhF-rpl32_p1 ndhF-rpl32 1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 20 24 55.0 33.3 58.1 57.3 0.0 0.0 4.844538 104245 104995 825-1003 1.000000 50.5 ndhF-rpl32_p2 ndhF-rpl32 2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 104245 104995 797-956 0.909091 46.0 ndhF-rpl32_p3 ndhF-rpl32 3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 104245 104995 798-957 0.909091 46.0 ndhF-rpl32_p4 ndhF-rpl32 4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 104245 104995 799-958 0.909091 46.0 ndhF-rpl32_p5 ndhF-rpl32 5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 104245 104995 800-959 0.909091 46.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAGGGTTTTTCTCGGCAACA TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 58.2 57.9 0.0 0.0 0.353983 105176 105716 595-631 1.000000 85.5 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AGGGTTTTTCTCGGCAACAA TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 58.2 57.9 0.0 0.0 0.353983 105176 105716 594-630 1.000000 85.5 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAGGGTTTTTCTCGGCAACA AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 58.2 57.8 0.0 0.0 0.414710 105176 105716 588-624 1.000000 85.1 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AGGGTTTTTCTCGGCAACAA AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 58.2 57.8 0.0 0.0 0.414710 105176 105716 587-623 1.000000 85.1 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AGGGTTTTTCTCGGCAACAA GCTTGAGCACTGCTTCCTAA 20 20 45.0 50.0 58.2 58.2 0.0 4.7 0.416192 105176 105716 608-644 1.000000 84.8