Hotspot

ycf1

SSC · 123234-128561 · 5328 bp

Back to Orychophragmus

Pi 0.00701

MarkerSeek score 43.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 92
pi: 0.00701
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGTCCCCAAACCGTTAAA	TGCGTTCCCAAAGACGTAAA	50.0	45.0	58.0	58.0	612 bp	1.0	81.0
ycf1_p2	AGTCGAGCCTTCTGAGATGA	TGCGTTCCCAAAGACGTAAA	50.0	45.0	57.9	58.0	556 bp	1.0	80.2
ycf1_p3	CCAGTCCCCAAACCGTTAAA	ATGCGTTCCCAAAGACGTAA	50.0	45.0	58.0	57.8	613 bp	1.0	80.1
ycf1_p4	CCAGTCCCCAAACCGTTAAA	GGAGCCTGGATTTCGTTCAA	50.0	50.0	58.0	58.2	791 bp	1.0	79.9
ycf1_p5	CCAGTCCCCAAACCGTTAAA	AAATGTACCAACGGAGCCTG	50.0	50.0	58.0	58.2	803 bp	1.0	79.8