Hotspot

ccsA

SSC · 113812-114798 · 987 bp

Back to Orychophragmus

Pi 0.00493

MarkerSeek score 54.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 12
pi: 0.00493
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCTAAAATCGGGTAAAACCTAGT	CAAAAGGTTCTTGATGATTTACT	34.8	30.4	55.1	53.3	1159-1172 bp	1.0	40.9
ccsA_p2	TCTAAAATCGGGTAAAACCTAGT	AGGTTCTTGATGATTTACTACT	34.8	31.8	55.1	52.2	1155-1168 bp	1.0	40.9
ccsA_p3	AAAATCGGGTAAAACCTAGT	CAAAAGGTTCTTGATGATTTACT	35.0	30.4	52.0	53.3	1156-1169 bp	1.0	40.9
ccsA_p4	AAAATCGGGTAAAACCTAGT	AGGTTCTTGATGATTTACTACT	35.0	31.8	52.0	52.2	1152-1165 bp	1.0	40.9
ccsA_p5	TCTAAAATCGGGTAAAACCTAGT	AAGGTTCTTGATGATTTACTACT	34.8	30.4	55.1	53.1	1156-1169 bp	1.0	40.9