Hotspot

rpl32-trnL

SSC · 112929-113618 · 690 bp

Back to Orychophragmus

Pi 0.0171

MarkerSeek score 59.10

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 690
variable_sites: 29
pi: 0.0171
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TTGGACATCGTTGAAAGCGA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	725-814 bp	1.0	85.5
rpl32-trnL_p2	TTGGACATCGTTGAAAGCGA	TGCAGACTGAGGCATTTTGT	45.0	45.0	58.1	58.0	1262-1352 bp	1.0	85.3
rpl32-trnL_p3	TTGGACATCGTTGAAAGCGA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	718-807 bp	1.0	85.0
rpl32-trnL_p4	TTGGACATCGTTGAAAGCGA	TAACCACTGGGACTGGAGAG	45.0	55.0	58.1	58.1	1295-1385 bp	1.0	84.9
rpl32-trnL_p5	TTGGACATCGTTGAAAGCGA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	723-812 bp	1.0	84.9