Hotspot

ndhF-rpl32

SSC · 111916-112769 · 854 bp

Back to Orychophragmus

Pi 0.0193

MarkerSeek score 64.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 854
variable_sites: 39
pi: 0.0193
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	47.6	40.0	57.6	55.5	946-964 bp	1.0	53.8
ndhF-rpl32_p2	TGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	45.0	40.0	56.3	55.5	945-963 bp	1.0	52.0
ndhF-rpl32_p3	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGAT	47.6	38.1	57.6	56.1	946-964 bp	1.0	50.2
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGG	ACGAATACGCTTTTTCGAGA	50.0	40.0	56.0	55.5	946-964 bp	1.0	49.4
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGGAT	ACGAATACGCTTTTTCGAGA	45.5	40.0	58.0	55.5	946-964 bp	1.0	49.1