Hotspot

trnR-trnN

IRb · 107665-108280 · 616 bp

Back to Orychophragmus

Pi 0.00346

MarkerSeek score 56.90

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 616
variable_sites: 5
pi: 0.00346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	748-757 bp	1.0	79.8
trnR-trnN_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	785-794 bp	1.0	79.2
trnR-trnN_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	789-798 bp	1.0	79.0
trnR-trnN_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	699-708 bp	1.0	78.7
trnR-trnN_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	826-835 bp	1.0	78.4