Hotspot

rpl36-rps8

LSC · 78809-79288 · 480 bp

Back to Orychophragmus

Pi 0.0148

MarkerSeek score 60.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 15
pi: 0.0148
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	607-617 bp	1.0	78.2
rpl36-rps8_p2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	606-616 bp	1.0	78.2
rpl36-rps8_p3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	606-616 bp	1.0	78.1
rpl36-rps8_p4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	605-615 bp	1.0	78.1
rpl36-rps8_p5	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	535-545 bp	1.0	77.8