Hotspot

rpl20-rps12

LSC · 67876-68658 · 783 bp

Back to Orychophragmus

Pi 0.00506

MarkerSeek score 57.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 11
pi: 0.00506
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	878-896 bp	1.0	80.9
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	892-910 bp	1.0	80.6
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	883-901 bp	1.0	80.4
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	822-840 bp	1.0	80.4
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	897-915 bp	1.0	80.0