Hotspot

rbcL-accD

LSC · 55826-56505 · 680 bp

Back to Orychophragmus

Pi 0.00846

MarkerSeek score 54.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 16
pi: 0.00846
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CACTACCAGGTGTTTTGCCT	GCTTTATTTAGCCCGCCTCT	50.0	50.0	58.0	58.0	1068-1097 bp	1.0	82.5
rbcL-accD_p2	TCACTACCAGGTGTTTTGCC	GCTTTATTTAGCCCGCCTCT	50.0	50.0	58.0	58.0	1069-1098 bp	1.0	82.4
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	GCTTTATTTAGCCCGCCTCT	45.0	50.0	58.0	58.0	1292-1321 bp	1.0	82.0
rbcL-accD_p4	TCATGGTATGCACTTCCGTG	GCTTTATTTAGCCCGCCTCT	50.0	50.0	58.0	58.0	1259-1288 bp	1.0	82.0
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	GCTTTATTTAGCCCGCCTCT	50.0	50.0	58.0	58.0	1459-1488 bp	1.0	81.8