Hotspot

BZJ73_gt016-trnL

LSC · 45833-46638 · 806 bp

Back to Orychophragmus

Pi 0.0121

MarkerSeek score 59.40

Primer available yes

Label BZJ73_gt016-trnL

Feature Metadata

label_name: BZJ73_gt016-trnL
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 25
pi: 0.0121
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
BZJ73_gt016-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	865-867 bp	1.0	80.4
BZJ73_gt016-trnL_p2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	868-870 bp	1.0	79.7
BZJ73_gt016-trnL_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	897-899 bp	1.0	79.5
BZJ73_gt016-trnL_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	900-902 bp	1.0	78.7
BZJ73_gt016-trnL_p5	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	55.0	50.0	57.9	57.6	870-872 bp	1.0	78.4