Hotspot

psaA-ycf3

LSC · 41403-42100 · 698 bp

Back to Orychophragmus

Pi 0.00774

MarkerSeek score 65.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 15
pi: 0.00774
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	810-819 bp	1.0	80.8
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	827-836 bp	1.0	80.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	818-827 bp	1.0	78.8
psaA-ycf3_p4	AGAAATGACCGGGTTTAGCC	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	835-844 bp	1.0	78.7
psaA-ycf3_p5	AGCCCATTCCTCGAAAGAAG	ATTGGTTGACGATCACGAGG	50.0	50.0	57.6	58.0	811-820 bp	1.0	78.4