Hotspot

psbZ-trnG

LSC · 35557-36016 · 460 bp

Back to Orychophragmus

Pi 0.00969

MarkerSeek score 58.30

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 12
pi: 0.00969
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	AGTCTTCTTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	542-559 bp	1.0	73.6
psbZ-trnG_p2	AGTCTTCTTGGTGGGTATCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	542-559 bp	1.0	69.1
psbZ-trnG_p3	GTCTTCTTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	541-558 bp	1.0	69.1
psbZ-trnG_p4	GTCTTCTTGGTGGGTATCCTT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	541-558 bp	1.0	67.3
psbZ-trnG_p5	TGGATTAGTCTTCTTGGTGGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	548-565 bp	1.0	67.2