Hotspot

trnC-petN

LSC · 27445-28047 · 603 bp

Back to Orychophragmus

Pi 0.0143

MarkerSeek score 52.40

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 19
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCATGCGAGACTTACTA	50.0	50.0	58.1	58.2	659-690 bp	1.0	80.9
trnC-petN_p2	GTTTCTATCGTTTTGGCGGC	AGCCCATGCGAGACTTACTA	50.0	50.0	58.1	58.2	715-746 bp	1.0	80.4
trnC-petN_p3	TTCTATCGTTTTGGCGGCAT	AGCCCATGCGAGACTTACTA	45.0	50.0	58.3	58.2	713-744 bp	1.0	79.3
trnC-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCATGCGAGACTTACTA	50.0	50.0	58.4	58.2	677-708 bp	1.0	78.6
trnC-petN_p5	TTTCCCCAGTTCAAATCCGG	ATTAAACCAGCCCATGCGAG	50.0	50.0	58.1	58.6	667-698 bp	1.0	77.8