Hotspot

trnS-trnG

LSC · 7872-8537 · 666 bp

Back to Orychophragmus

Pi 0.0206
MarkerSeek score 74.00
Primer available yes
Label trnS-trnG

Feature Metadata

label_name
trnS-trnG
feature_type
igs
region
LSC
length_bp
666
variable_sites
33
pi
0.0206
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG_p1 ACGCTTTAATCCACTCAGCC AATCAAACCGAGAGACCCTT 50.0 45.0 58.3 56.5 761-788 bp 1.0 72.9
trnS-trnG_p2 ACGCTTTAATCCACTCAGCC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.3 56.5 760-787 bp 1.0 72.9
trnS-trnG_p3 CGCTTTAATCCACTCAGCCA AATCAAACCGAGAGACCCTT 50.0 45.0 58.3 56.5 760-787 bp 1.0 72.9
trnS-trnG_p4 CGCTTTAATCCACTCAGCCA ATCAAACCGAGAGACCCTTT 50.0 45.0 58.3 56.5 759-786 bp 1.0 72.9
trnS-trnG_p5 CGGAAGGGAAAGAGAGGGAT ATCAAACCGAGAGACCCTTT 55.0 45.0 58.5 56.5 825-852 bp 1.0 70.6