Hotspot

rps16-trnQ

LSC · 6248-6695 · 448 bp

Back to Orychophragmus

Pi 0.00809

MarkerSeek score 54.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 10
pi: 0.00809
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGGTGGCGGATCAAAACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	555-579 bp	1.0	82.0
rps16-trnQ_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	509-533 bp	1.0	81.8
rps16-trnQ_p3	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	519-543 bp	1.0	81.0
rps16-trnQ_p4	AAAGGTGGCGGATCAAAACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	556-580 bp	1.0	80.2
rps16-trnQ_p5	AGGTGGCGGATCAAAACATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.5	58.1	554-578 bp	1.0	78.4