Hotspot

ndhH

Genome · 102932-104107 · 1176 bp

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Pi 0.48

MarkerSeek score 79.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1176
variable_sites: 450
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TTCTTTGGCCTAGTTTTCGA	TGCCATAAGAAGTCCAAGCG	40.0	50.0	54.9	58.3	2219 bp	0.5	33.1
ndhH_p2	TTTCTTTGGCCTAGTTTTCGA	TGCCATAAGAAGTCCAAGCG	38.1	50.0	55.7	58.3	2220 bp	0.5	31.7
ndhH_p3	TCTTTGGCCTAGTTTTCGAT	TGCCATAAGAAGTCCAAGCG	40.0	50.0	54.7	58.3	2218 bp	0.5	31.2
ndhH_p4	CGATTAGAAGATTTGGCATGTGT	TGCCATAAGAAGTCCAAGCG	39.1	50.0	57.5	58.3	2202 bp	0.5	30.0
ndhH_p5	TCTTTGGCCTAGTTTTCGATT	TGCCATAAGAAGTCCAAGCG	38.1	50.0	55.5	58.3	2218 bp	0.5	29.9