Hotspot

ndhG

Genome · 106703-107278 · 576 bp

Back to Orthotrichum

Pi 0.415

MarkerSeek score 81.50

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 576
variable_sites: 231
pi: 0.415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TTGCCTGCGAAGTATGTGTT	ACATCCATCCCAAGGTACCA	45.0	50.0	58.1	58.0	1649 bp	0.5	59.0
ndhG_p2	ATTGCCTGCGAAGTATGTGT	ACATCCATCCCAAGGTACCA	45.0	50.0	57.9	58.0	1650 bp	0.5	58.9
ndhG_p3	TTGCCTGCGAAGTATGTGTT	TCCAATAGCAGCTTCAGCAG	45.0	50.0	58.1	58.0	1323 bp	0.5	58.9
ndhG_p4	ATTGCCTGCGAAGTATGTGT	TCCAATAGCAGCTTCAGCAG	45.0	50.0	57.9	58.0	1324 bp	0.5	58.8
ndhG_p5	TGCGAAGTATGTGTTCGTGT	ACATCCATCCCAAGGTACCA	45.0	50.0	57.9	58.0	1644 bp	0.5	58.7