Hotspot

ndhE

Genome · 107308-107610 · 303 bp

Back to Orthotrichum

Pi 0.372

MarkerSeek score 77.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 103
pi: 0.372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGGTTGCATTAATAGGCGCT	ACATCCATCCCAAGGTACCA	45.0	50.0	57.9	58.0	687 bp	0.5	59.4
ndhE_p2	GTAGCTGCCGCACAAATTTT	ACATCCATCCCAAGGTACCA	45.0	50.0	57.9	58.0	1006 bp	0.5	59.3
ndhE_p3	TGGTTGCATTAATAGGCGCT	TTCGCATCGTTTACAACCCA	45.0	45.0	57.9	58.1	750 bp	0.5	58.6
ndhE_p4	GTAGCTGCCGCACAAATTTT	TTCGCATCGTTTACAACCCA	45.0	45.0	57.9	58.1	1069 bp	0.5	58.5
ndhE_p5	AGCCCGCCGAGAAAATTTAA	ACATCCATCCCAAGGTACCA	45.0	50.0	58.2	58.0	659 bp	0.5	58.5