Hotspot

ndhD

Genome · 108094-109593 · 1500 bp

Back to Orthotrichum

Pi 0.439

MarkerSeek score 80.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1500
variable_sites: 561
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGTACCTTGGGATGGATGT	GAACCCACGATCTCCATCAC	50.0	55.0	58.0	58.1	2262 bp	0.5	59.4
ndhD_p2	TGGTACCTTGGGATGGATGT	ATAAGCCGCCATGGTGAAAT	50.0	45.0	58.0	57.9	2095 bp	0.5	59.3
ndhD_p3	CTGCTGAAGCTGCTATTGGA	GAACCCACGATCTCCATCAC	50.0	55.0	58.0	58.1	2588 bp	0.5	59.3
ndhD_p4	CTGCTGAAGCTGCTATTGGA	ATAAGCCGCCATGGTGAAAT	50.0	45.0	58.0	57.9	2421 bp	0.5	59.2
ndhD_p5	TGGTACCTTGGGATGGATGT	CTAACGCATCTCGGTTCGAA	50.0	50.0	58.0	58.1	2045 bp	0.5	59.1