Hotspot

chlL

Genome · 95102-95977 · 876 bp

Back to Orthotrichum

Pi 0.467

MarkerSeek score 82.40

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 876
variable_sites: 371
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	CGCAATCAAGCAGGAAAAGG	TAGTTTTGGTGCCATGCCTT	50.0	45.0	58.0	58.0	1627 bp	0.5	59.8
chlL_p2	AGTGGGAAGTCGGGAAGTTA	TAGTTTTGGTGCCATGCCTT	50.0	45.0	58.0	58.0	1505 bp	0.5	59.8
chlL_p3	TAGTGGGAAGTCGGGAAGTT	TAGTTTTGGTGCCATGCCTT	50.0	45.0	58.0	58.0	1506 bp	0.5	59.8
chlL_p4	CGCAATCAAGCAGGAAAAGG	CTGGACAACGATGTGCCATA	50.0	50.0	58.0	58.0	1736 bp	0.5	59.7
chlL_p5	CGCAATCAAGCAGGAAAAGG	TGGACAACGATGTGCCATAG	50.0	50.0	58.0	58.0	1735 bp	0.5	59.7