Hotspot

rpl21-ndhF

Genome · 110993-111400 · 408 bp

Back to Orthotrichum

Pi 0.406

MarkerSeek score 79.00

Primer available yes

Label rpl21-ndhF

Feature Metadata

label_name: rpl21-ndhF
feature_type: igs
region: Genome
length_bp: 408
variable_sites: 154
pi: 0.406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-ndhF_p1	CTGTTCACCTCCGGTTTCAA	GGAAACTGAGCCGATTTTGC	50.0	50.0	58.0	58.0	1175 bp	0.5	59.6
rpl21-ndhF_p2	CTGTTCACCTCCGGTTTCAA	GAGGAACAAAAGAAGCGCAC	50.0	50.0	58.0	58.0	1146 bp	0.5	59.4
rpl21-ndhF_p3	ACCTCCGGTTTCAATGATGG	GGAAACTGAGCCGATTTTGC	50.0	50.0	57.9	58.0	1169 bp	0.5	58.8
rpl21-ndhF_p4	TCCGGTTTCAATGATGGCAT	GGAAACTGAGCCGATTTTGC	45.0	50.0	57.9	58.0	1166 bp	0.5	58.7
rpl21-ndhF_p5	ACCTCCGGTTTCAATGATGG	GAGGAACAAAAGAAGCGCAC	50.0	50.0	57.9	58.0	1140 bp	0.5	58.7